Fabrizio obtained his medical degree from the University of Padua, where he studied under the famous anatomist Gabriele Falloppio (1523–1562), who in turn had been a disciple of Vesalius. His best-known medical work is De venarum ostiolis (On the Venous Valves), published originally as an unbound folio pamphlet for the use of students, who were encouraged to bind it along with other Fabrizio’s publications printed in the same format. It consists of twenty-three pages of text and eight engraved plates, including the depiction of the outer anatomy of the veins of the forehand, which Harvey would adapt to illustrate his Exercitatio anatomica de motu cordis et sanguinis in animalibus.